Early detection and reliable screening are of vital importance in improving the survival rate. The 5-year relative GC survival rate of all Surveillance, Epidemiology, and End Results (SEER) stages combined is approximately 32%. pylori) infection and cancer-associated dietary habits. East Asia is recognized as a high-risk region probably due to the high incidence of Helicobacter pylori ( H. Gastric cancer (GC) is the fourth most common cancer in the world and causes approximately 738,000 deaths each year, making it the third leading cause of cancer-related death worldwide ( 1– 4).
The zinc finger protein CTCF regulates ECM-related genes, thereby promoting GC cell growth and migration. Collectively, this ECM-related 7-gene signature provides a novel insight for survival prediction among GC patients. Silencing CTCF or COL1A1/COL1A3 markedly inhibited cell growth and migration in the metastatic GC cell line BGC823. Furthermore, CTCF regulated COL1A1 and COLA31 expression in vitro. A comparison of human GC cell lines with high or low expression of ECM-related genes revealed different levels of tumor aggressiveness, suggesting the cancer development-promoting functions of ECM-related genes. In addition, CTCF was also correlated with the Wnt signaling pathway. CTCF binding sites also overlapped with histone activation markers, indicating direct regulation. Interestingly, putative CCCTC-binding factor (CTCF) regulatory elements were identified within the promoters of these ECM-related genes and were confirmed by chromatin immunoprecipitation sequencing (ChIP-Seq). We combined different gene expression datasets and filtered a 7-gene signature related to the extracellular matrix (ECM), which also exhibited significant prognostic value in GC patients. We aim to describe clinically relevant molecular classifications of GC that incorporate heterogeneity and provide useful clinical information. Gastric cancer (GC), a leading cause of cancer-related death, is a heterogeneous disease.
4Department of Molecular and Translational Science, Monash University, Clayton, VIC, Australia.3Centre for Innate Immunity and Infectious Diseases, Hudson Institute of Medical Research, Clayton, Victoria, Hudson Institute of Medical Research, Clayton, VIC, Australia.2Department of Sports Medicine, Huashan Hospital, Fudan University, Shanghai, China.1Faculty of Medical Laboratory Science, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.Chenbin Liu 1†, Linyi Deng 1†, Jinrong Lin 2, Jianjun Zhang 1, Shu Huang 1, Jinglin Zhao 1, Peipei Jin 1, Peiqing Xu 1, Peihua Ni 1, Dakang Xu 1*, Le Ying 1,3,4* and Yiqun Hu 1*
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